PCR amplification of MSP1_19. Gels were run for MSP1_19. The results of the PCR amplification of MSP1_19 are shown in figure 5. From the gel one can see that the MSP1_19 DNA segment is present in all four wells. Wells two and three contain the MSP1_19 DNA segment amplified using forward 3 and reverse 1 primers (see table 1). This MSP1_19 DNA segment was designed to have a basepair length of 291. The gel shows the band almost even with the 298 basepair band within the ladder, this is exactly where a 291 basepair segment is expected to be. Wells four and five contain the MSP1_19 DNA segment amplified using forward 4 and reverse 1 primers (see table 1). This primer set was designed to have a 685 basepair length amplification. The banding shows another successful amplification, because it was in between the 506 and 1018 basepair lengths shown on the ladder. This gel shows that the following steps were successful: amplification of purified genomic DNA, PCR, agarose gel electrophoresis, and PCR purification of DNA. The readings for the PCR amplification of MSP1_19 were quite successful (Table 5). The nanograms per microliter of DNA had fair numbers. The samples varied, but overall had decent results. The 260/280 content was also very good—readings were within a maximum of a .05 range.

PCR amplification of AMA1 and MSP2. Gels were ran for AMA1 and MSP2. Due to a time constraint, both AMA1 and MSP2 were not ligated. The results of the PCR amplification of AMA1 and MSP2 is shown in figure 6. Gel 2 depicts a positive amplification of MSP2 and AMA1 (Figure 6). The MSP2 primer set was designed to amplify a 601 basepair portion of MSP2. As the gel shows, the primer set was successful in the amplification of MSP2, in that it was right above the 506 basepair band within the ladder. AMA1 had an excellent gel reading as well. The AMA1 primer set was designed to amplify a 1,441 basepair segment of AMA1. The AMA1 bands were in the correct area of the gel, below the ladder basepair length of 1,636. This gel shows that the following steps were successful: amplification of purified genomic DNA, PCR, agarose gel electrophoresis, and PCR purification of DNA.

Part I: Ligation of pGEM-T Easy vector and MSP1_19 inserts, Transformation, and Plasmid Purification. The results of the ligation and transformation steps are depicted in the gel shown in figure 7. The gel shows that the ligation, transformation, and plasmid purification were successful due to the presence of a bands within the 298 basepair region. Wells 2, 3,4, 6, and 7 all have MSP1_19 F3 DNA segments present. The bands above the MSP1_19 F3 bands are of the correct size for the pGEM-T Easy vector. Figure 8 depicts a positive presence of MSP1_19 F4, 685 bp in length, in wells three and four. The readings for the pGEM-T Easy vector with the MSP1_19 insert are seen in table 6. NanoDrop readings were positive for the most part (Table 6). All samples, except for 3C, had great nucleic acid content and an excellent 260/280 ratio. The reasoning for the -80.0 nanograms per microliter nucleic acid content, is due to the fact it was probably contaminated with ethanol. This is the same reasoning for the low 260/280 ratio. The reason 2C has such a low nucleic acid content is because part of the sample was spilled.

Part II: Ligation of pSAT vector and MSP1_19 inserts, Transformation, and Plasmid Purification. The image in figure 9 shows that the ligation of the MSP1_19 F4 DNA segment into the pSAT vector was unsuccessful. All steps following the ligation were also unsuccessful as a result of the failed ligation. The gel in figure 9 and 10 show a lack of banding in the 698 basepair region, where we would expect to see bands if the MSP1_19 F4 DNA segment were present. The readings for the pSAT vector cassettes with the MSP1_19 F4 inserts are seen in table 7.The NanoDrop Readings conducted on the pSAT vectors were very good (Table 7). Once again, nucleic acid content and the 260/280 ratio had great readings.